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McKelvey School of Engineering

Brown School

BME Seminar: Yifan Dai, PhD

Thursday, February 23, 2023 | 10:00 AM - 11:00 AM

Uncas A. Whitaker Hall, 218
6760 Forest Park Pkwy, St. Louis, MO 63105, USA

Presenting on “A New Phase of Biological Controls- A design framework for programmable synthetic biomolecular condensates and the mechanisms of a functional liquid-liquid interface”

Yifan Dai, PhD, Department of Biomedical Engineering, Duke University, will speak on Thursday, February 23, 2023 at 10:00 am CST in Whitaker 218.

Abstract: A fundamental question in nature is how the cellular processes are organized with sequential and spatial precision in a dynamic and densely packed environment. Evidence is now mounting that biomolecular condensation, a demixing process mediated by phase separation coupled to percolation, dictates the organization principles of cellular biochemistry. From the perspective of synthetic biology, programmable condensation in living cells represents a new fundamental capability for biological design, going beyond the current engineering capability of lock-and-key interactions. In the first part of the talk, I will introduce a rational design strategy of synthetic intrinsically disordered proteins toward functional synthetic biomolecular condensates for cellular controls in bacteria and human cells. I will demonstrate the applications of synthetic condensates on four distinct cellular processes: cell division, transcription, translation, and modulation of protein circuits, providing a toolbox for orthogonal central dogma.

In the second part of the talk, I will dig into the physical chemistry principles of condensate microenvironments, by which condensates can encode unique electrochemical features at its liquid-liquid interface. I will introduce a theoretical framework we developed for condensate interface, which allows us to understand the density transition process of condensate formation from the perspective of electrochemistry. I will then discuss our experimental discoveries on the fundamental electrochemical properties of liquid-liquid interface and how these features can regulate cellular processes. These discoveries open new directions of condensate research and provide answers for many previously unexplained biological activities of biomolecular condensates.
Registration is required to attend virtually. Please register.

Event Type

Seminar/Colloquia

Schools

McKelvey School of Engineering

Topic

Science & Technology, Medicine & Health

Department
Biomedical Engineering
Hashtag

#WashUBME

Event Contact

Mimi Hilburg | mhilburg@wustl.edu

Speaker Information

Bio: Yifan Dai is a postdoc associate at the Department of Biomedical Engineering at Duke University, co-advised by Prof. Ashutosh Chilkoti and Prof. Lingchong You. He obtained his B.S. and Ph.D. in 2017 and 2020 both in Chemical and Biomolecular Engineering from Case Western Reserve University. Since his undergraduate, he had worked on engineering strategies to integrate synthetic biology with electrochemistry to enable translation of biological signals into electricity for biosensing applications, which have led to multiple licensed technologies. In his postdoc, he has worked on 1) uncovering the principles of biomolecular phase transitions for engineering biology and 2) establishing the theoretical framework of condensate microenvironments and liquid-liquid interface.

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